Yangming Wang, Ph.D.

Professor

PrincipalInvestigator

Laboratory of Stem Cells and Noncoding RNAs 

Institute of Molecular Medicine,

Peking University, Beijing, China100871

E-mail: yangming.wang@pku.edu.cn

Phone: 86-10-62766945

 

Professor Wang received his B.S. degree in Biotechnology from Peking University in 2000. He then joined Dr. Scott K. Silverman's laboratory to study nucleic acid biochemistry at the University of Illinois at Urbana-Champaign, where he obtained his Ph.D. degree in Biochemistry in 2006. After that, he became a postdoctoral fellow studying microRNAs and embryonic stem cells in Dr. Robert H. Blelloch's laboratory at the Unviersity of California, San Francisco. He became a principle investigator at the Institute of Molecular Medicine in 2011.

 

Selected Publications:

 

PEER REVIEWED ORIGINAL RESEARCH PAPERS (Total citations >2000)

29. ^#Wang, X.W., ^#Hu, L.F., Hao, J., Liao, L.Q., Chiu, Y.T., Shi, M.and *Wang Y. (2019) A microRNA-inducible CRISPR-Cas9 platform serves as a microRNA sensor and cell-type-specific genome regulation tool. Nature Cell Biology 21, 522-530.

28. ^#Li, Y.P., ^#Duan, F.F., Zhao, Y.T., Gu, K.L., Liao, L.Q., Su, H.B., Hao, J., Zhang, K., Yang, N. and *Wang Y. (2019) A TRIM71 binding long noncoding RNA Trincr1 represses FGF/ERK signaling in embryonic stem cells. Nature Communications 10, 1368.

27. Guo, W.T. and *Wang Y. (2019) Dgcr8 knockout approaches to understand microRNA functions in vitro and in vivo. Cellular and Molecular Life Sciences doi: 10.1007/s00018-019-03020-9.

26. Wu, D.R., Gu, K.L., Yu, J.C., Fu, X., Wang, X.W., Guo, W.T., Liao, L.Q., Zhu, H., Zhang, X.S., *Hui J and *Wang Y. (2018) Opposing roles of miR-294 and MBNL1/2 in shaping the gene regulatory network of embryonic stem cells. EMBO Reports 19, e45657.

25. Bao, X., Guo, X., Yin, M., Tariq, M., Lai, Y., Kanwal, S., Zhou, J., Li, N., Lv, Y., Pulido-Quetglas, C., Wang, X., Ji, L., Khan, M.J., Zhu, X., Luo, Z., Shao, C., Lim, D.H., Liu, X., Li, N., Wang, W., He, M., Liu, Y.L., Ward, C., Wang, T., Zhang, G., Wang, D., Yang, J., Chen, Y., Zhang, C., Jauch, R., Yang, Y.G., Wang, Y., Qin, B., Anko, M.L., Hutchins, A.P., Sun, H., Wang, H., Fu, X.D., Zhang, B., Esteban, M.A. (2018) Capturing the interactome of newly transcribed RNA. Nature Methods 15，213-220.

24. Chen, X., Wang, L., Huang, R., Qiu, H., Wang, P., Wu, D., Zhu, Y., Ming, J., Wang, Y., Wang, J. and *Na, J. (2018) Dgcr8 deletion in the primitive heart uncovered novel microRNA regulating the balance of cardiac-vascular gene program. Protein & Cell doi: 10.1007/s13238-018-0572-1.

23. Jiang, L., Shao, C., Wu, Q.J., Chen, G., Zhou, J., Yang, B., Li, H., Gou, L.T., Zhang, Y., Wang, Y., Yeo, G.W., Zhou, Y., Fu, X.D. (2017) NEAT1 scaffolds RNA-binding proteins and the Microprocessor to globally enhance pri-miRNA processing. Nature Structural and Molecular Biology 24，816-824.

22. Wang, X.W., Hao, J., Guo, W.T., Liao, L.Q., Huang, S., Guo, X., Bao, X., Esteban, M.A. and *Wang, Y. (2017) DGCR8-independent stable microRNA expression strategy reveals important functions of miR-290 and miR-183~182 families in mouse embryonic stem cells. Stem Cell Reports 9，1618-1629.

21. Wei, W., Liu, Y., Zhang, Q., Wang, Y., Zhang, X. and *Zhang H. (2017) Danshen-Enhanced Cardioprotective Effect of Cardioplegia on Ischemia Reperfusion Injury in a Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Model. Artificial Organs 41:452-460.

20. ^#Gu, K.L., ^#Zhang, Q., ^#Yan, Y., ^#Li, T.T., Duan, F.F., Hao, J., Wang, X.W., Shi, M., Wu, D.R., Guo, W.T., *Wang, Y. (2016) Pluripotency Associated miR-290/302 Family of microRNAs Promote the Dismantling of Naive Pluripotency. Cell Research 26, 350-366.

19. ^#Cao, Y., ^#Guo, W.T., Tian, S., He, X., Wang, X.W., Liu, X., Gu, K.L., Ma, X., Huang, D., Cai, Y.P., Zhang, H., *Wang, Y. and *Gao, P. (2015) miR-290/371-Mbd2-Myc Circuit Regulates Glycolytic Metabolism to Promote Pluripotency. EMBO Journal 34, 609-623.

18. ^#Yan, Y., ^#Yang, X., Li, T.T., Gu, K.L., Hao, J., Zhang, Q., *Wang, Y. (2017) Significant Differences of Function and Expression of microRNAs between Ground State and Serum-Cultured Pluripotent Stem Cells. Journal of Genetics and Genomics 44，179-189.

17. Guo, W.T., Wang, X.W., Yan, Y.L., Li, Y.P., Yin, X., Zhang, Q., Melton, C., Shenoy, A., Reyes, N.A., Oakes, S.A., *Blelloch, R. and *Wang Y. (2015) Suppression of Epithelial-Mesenchymal Transition and Apoptotic Pathways by miR-294/302 Synergistically Blocks let-7-induced Silencing of Self-renewal in Embryonic Stem Cells. Cell Death and Differentiation 22, 1158-1169.

16. ^#Ma, Y., ^#Yao, N., ^#Liu, G., Dong, L., Wang, F., Wang, B., Dong, H., Wei, X., Zhang, M., Ji, S., Liu, Y., Zhang, J., Wang Y, *Huang Y. and *Yu J. (2015) Functional Screen Reveals Essential Roles of miR-27a/24 in Differentiation of Embryonic Stem Cells. EMBO Journal 34, 361-378.

15. *^#Wang, Y., ^#Melton, C., Li, Y.P., Shenoy, A., Zhang, X.X., Subramanyam, D. and *Blelloch, R. (2013) miR-294/miR-302 Promotes Proliferation, Suppresses G1-S Restriction Point, and Inhibits ESC Differentiation through Separable Mechanisms. Cell Reports 4, 99-109.

14. Wang, Y., Baskerville, S., Shenoy, A., Babiarz, J.E., Baehner, L. and *Blelloch, R. (2008) Embryonic Stem Cell Specific microRNAs Regulate the G1/S Transition and Promote Rapid Proliferation. Nature Genetics 40, 1478-1483. (Highlighted in Nature Reports Stem Cells ; in News and Views in Nature Genetics , 2008, 40, 1391-1392; and in Cell Stem Cell , 2009, 4, 9-10)

13. Babiarz, J.E., Ruby, J.G., Wang, Y., *Bartel, D.P. and *Blelloch, R. (2008) Endogenous shRNAs, siRNAs, and Other Microprocessor-independent, Dicer-dependent Small RNAs in Mouse ES cells. Genes & Development 22, 2773-2785.

12. Wang, Y., Medvid, R., Melton, C., Jaenisch, R. and *Blelloch, R. (2007) DGCR8 is Essential for microRNA Biogenesis and Silencing of Embryonic Stem Cell Self-Renewal. Nature Genetics 39, 380-385.

11. Wang, Y. and *Silverman, S.K. (2006) Experimental Tests of Two Proofreading Mechanisms for 5′-Splice Site Selection. ACS Chemical Biology 1, 316-324.

10. Wang, Y. and *Silverman, S.K. (2006) Efficient RNA 5'-Adenylation By T4 DNA Ligase to Facilitate Practical Applications. RNA 12, 1142-1146.

9. Zelin, E., Wang, Y. and *Silverman, S.K. (2006) Adenosine is Inherently Favored as the Branch-Site RNA Nucleotide in a Structural Context that Resembles Natural RNA Splicing. Biochemistry 45, 2767-2771.

8. Wang, Y. and *Silverman, S.K. (2006) A General Two-Step Strategy To Synthesize Lariat RNAs. RNA 12, 313-321.

7. Wang, Y. and *Silverman, S.K. (2005) Efficient One-Step Synthesis of Biologically Related Lariat RNAs by a Deoxyribozyme. Angewandte Chemie International Edition 44, 5863-5866. (Highlighted in Nature Chemical Biology, 2005, 1, 195)

6. Pratico, E.D., Wang, Y. and *Silverman, S.K. (2005) A Deoxyribozyme That Synthesizes 2’,5’-Branched RNA With Any Branch-Site Nucleotide. Nucleic Acids Research 33, 3503-3512.

5. Wang, Y. and *Silverman, S.K. (2005) Directing the Outcome of Deoxyribozyme Selections to Favor Native 3'-5' RNA Ligation. Biochemistry 44, 3017-3023.

4. Wang, Y. and *Silverman, S.K. (2003) Characterization of Deoxyribozymes that Synthesize Branched RNA. Biochemistry 42, 15252-15263.

3. Wang, Y. and *Silverman, S.K. (2003) Deoxyribozymes that Synthesize Branched and Lariat RNA. Journal of the American Chemical Society 125, 6880-6881.

2. Flynn-Charlebois, A., Wang, Y., Prior, T.K., Rashid, I., Hoadley, K.A., Coppins, R.L., Wolf, A.C. and *Silverman, S.K. (2003) Deoxyribozymes with 2'-5' RNA Ligase Activity. Journal of the American Chemical Society 125, 2444-2454.

1. Zhen, W., Wang, Y., Ding, W., Chen, X., Hu, Y. and *Lin, Z. (2001) Cre/loxP-mediated Deletion of Transgene in Transgenic Tobacco. Journal (natural sciences) of Peking University 37, 477-482.

INVITED REVIEWS

5. Hao, J., Duan, F.F. and *Wang, Y. (2017) MicroRNAs and RNA binding protein regulators of microRNAs in the control of pluripotency and reprogramming. Current Opinion in Genetics & Development 46, 95-103.

4. Li, Y.P. and *Wang, Y. (2015) Large Noncoding RNAs are Promising Regulators in Embryonic Stem Cells. Journal of Genetics and Genomics 42, 99-105.

3. Guo, W.T., Wang, X.W. and *Wang, Y. (2014) Micro-management of Pluripotent Stem Cells. Protein & Cell 5, 36-47.

2. Wang, Y. and *Blelloch, R. (2011) Cell Cycle Regulation by microRNAs in Stem Cells. Results and Problems in Cell Differentiation 53, 459-472.

1. Wang, Y. and *Blelloch, R. (2009) Cell Cycle Regulation by microRNAs in Embryonic Stem Cells. Cancer Research 69, 4093-4096.